Delftia deserti sp. nov., isolated from a desert soil sample.

Abstract

A Gram-staining negative, short rod, motile, light brownish-pigmented bacterial strain, designated YIM Y792(T), was isolated from a soil sample taken from Turpan desert in Xinjiang Uyghur Autonomous Region, north-western China. Phylogenetic analysis indicated that strain YIM Y792(T) belongs to the genus Delftia. Strain YIM Y792(T) shared highest 16S rRNA gene sequence similarities with Delftia lacustris DSM 21246(T) (93.96%), Delftia tsuruhatensis NBRC 16741(T) (93.74%), and Delftia acidovorans NBRC 14950(T) (93.62%). Growth of the strain YIM Y792(T) was found to occur at 20-45°C (optimum at 30°C), pH 6.0-9.0 (optimum at pH 7.0), and salinities of 0-3.0% NaCl (optimum at 1.0%). The new bacterium exhibits typical chemotaxonomic features of the genus Delftia with ubiqinone-8 (Q-8) as the predominant quinone and C16:0, Summed feature 3, Summed Feature 8 as major fatty acids (>10%). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, glycolipid, two unidentified phospholipids and one unidentified lipid. The G+C content of the genomic DNA of strain YIM Y792(T) was found to be 70.3mol%. The DNA-DNA relatedness values between strain YIM Y792(T) and D. lacustris DSM 21246(T), D. tsuruhatensis NBRC16741(T), D. acidovorans NBRC14950(T) were 35.5±2.0, 17.1±1.8, 26.2±2.0%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented here, we propose a novel species with the name Delftia desertisoli sp. nov. The type strain is YIM Y792(T) (=KCTC 42377(T)=JCM 30639(T)).