Delayed hypersensitivity in several strains of mice studied with six different tests

Abstract

Delayed hypersensitivity (tuberculin allergy) was induced in seven different strains of mice and detected by means of six different kinds of tests in order to compare both the tests and mouse strains under standardized conditions. The mice were sensitized by two weekly subcutaneous injections of living H37Ra tubercle bacilli suspended in water-in-oil emulsions, and they were tested three weeks after the first vaccination. C57BL, C57BR, C57L, DBA, and CF#1 mouse strains were not essentially different, either in frequency or in intensity of hypersensitization. C3H mice appeared to be less frequently sensitized than mice of other strains but, when sensitive, reacted as strongly. Swiss Webster mice were sensitized as frequently but reacted somewhat more intensely. The six tests employed were divided into primary and secondary categories by reliability, sensitivity, versatility, and simplicity. Footpad, intracutaneous, and intramuscular tests made up the first category; lung density, body temperature, and systemic tests comprised the second. Some basic data concerning delayed hypersensitivity in the mouse as well as its detectability by the traditional skin test have been presented and discussed.