Delayed fertilization of oocytes following in vitro culture results in increased embryo aneuploidy

Abstract

Rescue ICSI (rICSI), the re-insemination of oocytes after failed fertilization or delayed ICSI (dICSI), the fertilization of oocytes following in vitro maturation from the GV or MI stage, are controversial assisted reproduction techniques. While some laboratories have reported pregnancies from rICSI and dICSI, it is generally accepted that the pregnancy rates are lower than traditional ART procedures and there may be an increased risk of chromosomal abnormalities. The intent of this study was to identify if dICSI results in an increased embryonic aneuploidy status.