Delatylus andersoni n. gen., n. sp. (Nematoda: Neotylenchidae) Isolated from White Pine (Pinus monticola) Lumber from USA and Intercepted in Ningbo, China.

Abstract

Three populations of neotylenchid nematodes were isolated in Ningbo, P. R. China, from white pine lumber (Pinus monticola) imported from the USA. The nematodes were morphologically intermediate between Hexatylus and Deladenus. The nematodes were molecularly characterized based on sequences of the rDNA small subunit 18S, large subunit 28S D2/D3, and internal transcribed spacer sequences. The phylogenetic inferences placed the nematodes with other neotylenchid nematodes, i.e., Fergusobia and Rubzovinema. Based on the morphology and phylogenetic analysis, this nematode is described herein as Delatylus andersoni n. gen., n. sp. The new genus/species is characterized by the female body habitus ranging from nonobese to semiobese and from straight to dorsally curved when heat relaxed, cephalic framework with six unequal sized lip sectors, lateral fields having 10 to 12 lines, 4 to 5 guide rings on the stylet, excretory pore posterior to the nerve ring, spermatheca diminished or absent, vulvar opening large, and relative proximity of vulva to the anus. Detailed morphological and molecular characterization of the new genus/species is presented along with the comparison of the related genera.