Dehydration induced hypoxia and its role on mitochondrial respiratory enzymes and oxidative stress responses in liver of Asian stinging catfish Heteropneustes fossilis

Abstract

In the present study, Water Deprived Condition (WPC, up to 18 h) induced hypoxia and altered oxidative stress (OS) physiology along with responses of respiratory chain enzyme in Heteropneustes fossilis are described . The body O2 saturation level in the fish was declined with respect to air exposure. Higher levels of lipid peroxidation and protein carbonylation were recorded in the tissue of fish exposed to 6 h of WPC stress. The regulation of the mitochondrial complex and antioxidant enzymes, small antioxidant molecules indicated that the fish can moderately survive up to 6 h of air exposure. Probably with the onset of metabolic depression, it can critically resist the dehydration stress up to 18 h. Although the activities of glutathione peroxidase and reductase were elevated, activities of antioxidant enzymes such as superoxide dismutase and catalase were insufficient to combat WPC induced ROS and OS generated under hypoxia. The small antioxidant molecules played a key role in elimination of ROS. The elevated complex II activity was probably responsible for resisting the complex I, II and IV mediated electron leakage events in mitochondria of the fish under WPC. The total H2O2 removing capacity was less under WPC while the total units of all calculated antioxidants were alleviated signifying an interesting mechanism of WPC induced OS in the fish.