Abstract
Protocorms of Cymbidium finlaysonianum cultured on MS agar medium without plant growth regulator were dehydrated on cryo-plates with PVS2, PVS3 and glycerol for 0, 30, 60, 90 and 120 min prior to being plunged into liquid nitrogen (LN) for 1 h. The results showed that treated controls (PVS2, PVS3 and glycerol without LN) gave lower survival when the exposure times were longer. The PVS2 and PVS3 exposure time for 60 min prior to plunging into LN gave the highest survival of 40 and 10%, respectively. There is no survival after cryopreservation when glycerol was used for dehydration. After that, cryopreserved protocorms developed into plantlets within 3 months of culture.
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