Dehalogenase enzyme activity of Bacillus sp. D1 isolated from pharmaceutical waste

Abstract

Dichloromethane (DCM) is widely used in the pharmaceutical industry as an intermediate solvent and active reagent. Most of the pharmaceutical industry waste contains of DCM which is a toxic compounds in the environment. Microorganism plays an important role in maintaining environmental ecosystems by degrading organic pollutants. Dichloromethane degrading microorganisms are known have dehalogenase enzyme activity. Bacillus sp. D1 isolated from pharmaceutical industry waste was used in this study. This study aims to determine the activity of Bacillus sp. D1 dehalogenase enzyme in various incubation time and pH condition. This research is an experimental study that used a completely randomized design with three replications. Bacillus sp. D1 was cultured in the mineral salt medium (MSM) added by DCM in rotary shaker 130 rpm until 7 days incubation. Evaluation of enzyme activity was carried out at various incubation times and pH conditions using the Bergman method. The best enzyme activity was 0.285 U/mL reached at 48 hours of incubation. The highest dehalogenase enzyme activity was 0.913 U/mL at pH 9. The activity of dehalogenase enzyme from Bacillus sp. D1 optimum at alkaline condition.