Degree of organ damage and inflammatory markers in sepsis mice models inducted by various doses of lipopolysaccharides

Abstract

Background Sepsis is a life-threatening disease, and animal models of sepsis are minimal. This study aims to find the optimal dose of LPS to make a sepsis mouse model by examining the presence of target organ damage. Methods This study used 30 mice divided into four groups. The control group (3 mice) injected 0.5 cc NaCl 0.9% intraperitoneally (i.p.). Group A (9 mice) was injected with lipopolysaccharides (LPS) 0.125 mg/kg B.W. i.p. given on the first and second day, group B (9 mice) was injected with LPS 0.15 mg/kg B.W. i.p. given on the first and second days, and group C (9 mice) was injected by LPS 0.3 mg/kg B.W. single dose i.p. On the third, fourth, and fifth days, the termination of each group of three mice and examination of the NF-κB, tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), alanine aminotransferase (ALT), the expression of NF-κB in the liver and kidneys, and histopathology in the liver and kidneys were conducted. Results NF-κB, C-reactive protein (CRP), alanine aminotransferase (ALT), NF-κB examinations, and tumor necrosis factor-α (TNF-α) in all treatment groups increased when compared with the control. The highest degree of histopathological features of the kidneys and liver and the results of immunohistochemistry examinations on the liver and kidneys were shown in group C. Conclusions The optimal dose of LPS to make a sepsis mouse model was 0.3 mg/kgB.W with the most severe target organ damage dan significant increased of inflammatory markers.