Abstract
The phosphodiesterase present in the culture medium of Micrococcus pyogenes var. aureus was purified and its action on tobacco mosaic virus nucleic acid was studied. In the complete digest nucleoside 3′-phosphates of both purines and pyrimidines and also several di-nucleotides were found. Its action on polyuridylic and cytidylic acids also resulted in the production of the corresponding nucleoside 3′-phosphates and dinucleotides. The enzyme was found to have no action on purine or pyrimidine cyclic nucleotides. The possible mode of its degradation of tobacco mosaic virus nucleic acid was discussed.
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