Abstract
Swainsonine is an indolizidine alkaloid that has been found in locoweeds and some fungi. Our previous study demonstrated that Arthrobacter sp. HW08 or its crude enzyme extract could degrade swainsonie efficiently. However, the mechanism of swainsonine degradation in bacteria remains unclear. In this study, we used label-free quantitative proteomics method based on liquid chromatography-electrospray ionization-tandem mass spectrometry to dissect the mechanism of swainsonine biodegradation by Arthrobacter sp. HW08. The results showed that 129 differentially expressed proteins were relevant to swainsonine degradation. These differentially expressed proteins were mostly related to the biological process of metabolism and the molecular function of catalytic activity. Among the 129 differentially expressed proteins, putative sugar phosphate isomerase/epimerase A1R5X7, Acetyl-CoA acetyltransferase A0JZ95, and nicotinamide adenine dinucleotide phosphate (NADP)-dependent alcohol dehydrogenase A1R6C3 were found to contribute to the swainsonine degradation. Notably, NADP-dependent alcohol dehyrodgenase A1R6C3 appeared to play a major role in degrading swainsonine, but not as much as Arthrobacter sp. HW08 did. Collectively, our findings here provide insights to understand the mechanism of swainsonine degradation in bacteria.
Highlights
Locoweed poisoning is a great threat to grass farming in the livestock industry [1]
We strain assay, we demonstrated that the nicotinamide adenine dinucleotide phosphate (NADP)‐dependent alcohol dehydrogenase
The The results showed that performedto toinvestigate investigatethe thedynamic dynamic changes degradation
Summary
Locoweed poisoning is a great threat to grass farming in the livestock industry [1]. Pathology studies showed that SW can inhibit cellular alpha-mannosidase and induce lysosomal accumulation of incompletely processed oligosaccharides as vacuoles in many cell types, including liver, renal, and cerebellar cells [5,6]. The pathological changes caused by SW directly or indirectly lead to the clinical symptoms of locoism, including birth defects, reproductive disorders, congestive heart failure, edema, growth retardation, and body weight loss [7,8]. Several methods have been used so far to prevent SW poisoning in livestock by either managing locoweeds or administering vaccines [9,10,11].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
