Abstract
The mutagenic effects of rutin and quercetin have aroused the interest of many investigators. To develop the microbial degradation of rutin, a thermophilic actinomycete, which could hydrolyze rutin, was isolated from compost soil. The taxonomical characteristics of this thermophilic actinomycete were examined and identified as Thermoactinomyces vulgaris PU18-2. After cultivation of T. vulgaris PU18-2 in the rutin-CYC medium for 60 h, the culture filtrate had a rutin-degrading ability, but the cell-free extract did not. There was no quercetin, rutinose, rhamnose, and glucose accumulated in the rutin hydrolysate of the culture filtrate. Both alpha-rhamnosidase and beta-glucosidase activities were not found in the culture filtrate of the T. vulgaris PU18-2 in the rutin-CYC medium. These results showed that the initial attack on rutin by the extracellular enzymes of T. vulgaris PU18-2 apparently was not through the glycosidase-mediated hydrolysis of glycosidic bond.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
