Abstract
The in vitro degradation of porcine and human synthetic calcitonin was measured in the presence of sera of different species (rat, pig, human) using a chromatoelectrophoresis method. Degradation is greater when heterologous sera are used. The degradation was not prevented by enzyme inhibition. Damage was greatest at acid pH and was not removed by heating of the serum. Ammonium sulfate precipitations and euglobulin separation of serum proteins did not result in the isolation of the factor responsible for the degradation of calcitonin. Mercaptoethanol prevented the damage from appearing which suggested that this damage was in part due to breakage of the disulfide bond.
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