Abstract
The aim of this research was to ascertain the efficacy of immobilized yeasts for the degradation of pyrene in soil. Pyrene degradation by individual yeasts and their mixtures was clearly enhanced when the yeasts were immobilized with chemical and physical entrapments, and cross-shaped beads from physical entrapment were more promising than spherical beads from chemical entrapment. Immobilized yeast mixture Candida tropicals Y 219 + Y 220 showed the best degradation of pyrene, exhibiting collaboration in pyrene removal. Scanning electron micrographs demonstrated good macroporous structure for mass transfer of substrates and immobilized cell growth.
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