Degradation of polycyclic aromatic hydrocarbon (pyrene) using novel fungal strain Coriolopsis byrsina strain APC5

Abstract

Coriolopsis byrsina strain APC5, white rot fungi was isolated from Surguja district of Chhattisgarh, India, which was able to degrade 96.1% of pyrene with the help of their ligninolytic activity. C. byrsina produced a significant amount of ligninolytic enzyme upto p < 0.0001 significant level in the pyrene containing mineral salt broth. Different physical parameters like pH (3.0–8.0), temperature (15–55 °C) and salinity (10 g L−1, 32 g L−1) were optimized for the efficient degradation. The highest degradation of pyrene and enzyme production was found on pH 6 at 25 °C temperature. The pyrene degradation was analyzed by High performance liquid chromatography and functional group modification of pyrene degraded metabolites were characterized by Fourier transform infrared spectroscopy and identified by Gas chromatography-mass spectroscopy. The metabolic intermediate compounds are identified, such as pyruvic acid, benzoic acid, benzoic acid 2-hydroxy pentyl ester, phenanthrene, pthalic acid diisopropyl ester, 4, 5 di hydroxy pyrene. Degradation of pyrene was also examined in in-vivo condition and 51.85% degradation was investigated in soil, On the basis of this study, C. byrsina strain APC5 can be used as potential pollutant degrader from the environment after successful field trial.