Degradation of novel mineral flotation reagent 8-hydroxyquinoline by superparamagnetic immobilized laccase: Effect, mechanism and toxicity evaluation

Abstract

• The effect of main constituents of MPW on 8-HQ degradation was evaluated. • Favorable 8-HQ degradation efficiency was achieved in real lead–zinc mine water. • The biocatalyst exhibited efficient recyclability and reusability. • Degradation predominantly generated various structural 8-HQ oligomers/polymers. • TOC and toxicity were effectively reduced with the degradation of 8-HQ. The environmental impact of the mining industry requires efficient and eco-friendly technologies to mitigate the presence of mineral flotation reagents (MFRs) in mineral processing wastewater (MPW) prior to their discharge into the environment. In this work, for the first time, a robust, easily separable and reusable biocatalyst, Fe 3 O 4 @SiO 2 -NH 2 -Lac, was used for the degradation of a novel mineral flotation reagent 8-hydroxyquinoline (8-HQ). Under optimized conditions, Fe 3 O 4 @SiO 2 -NH 2 -Lac achieved 89.2% 8-HQ degradation efficiency within 6 h. The effect of the main constituents of MPW on 8-HQ degradation, including metal ions, organic solvents, surfactant, metal chelator and flotation frother was evaluated. The Fe 3 O 4 @SiO 2 -NH 2 -Lac also displayed favorable degradation efficiency of 8-HQ in real lead–zinc mine water. The biocatalyst could be easily recovered and had a satisfactory reusability, retaining 64.5% of 8-HQ degradation efficiency in the sixth reaction cycle. Identification of intermediate products revealed that Fe 3 O 4 @SiO 2 -NH 2 -Lac mediated reaction predominantly generated various structural 8-HQ oligomers/polymers. A potential degradation pathway for 8-HQ was speculated as follows: Fe 3 O 4 @SiO 2 -NH 2 -Lac initially catalyzed the oxidation of 8-HQ to yield the corresponding reactive radical intermediates, which subsequently undergone self-coupling reaction via C − C and C − O − C covalent coupling at their ortho and/or para positions, finally forming oligomers and polymers. The inhibition assays of marine bacterium ( Vibrio fischeri ) demonstrated that the toxicity of 8-HQ and its intermediate products was effectively reduced after Fe 3 O 4 @SiO 2 -NH 2 -Lac treatment. The results of this study might present an alternative immobilized laccase-based clean biotechnology for the clean-up and detoxification of 8-HQ contaminated MPW.