Abstract
BackgroundMitochondria play a significant role in plant cytoplasmic male sterility (CMS). In our previous study, mitochondrial complex I genes, nad4, nad5, and nad7 showed polymorphisms between the transgenic CMS line M2BS and its wild type M2B. The sterility mechanism of the M2BS at cytological, physiological, biochemical, and molecular level is not clear.ResultsCytological observation showed that the anthers were light yellow, fissured, invalid in KI-I2, and full of irregularly typical abortion pollen grains in M2BS. Transmission electron microscopic (TEM) observation revealed no nucleus and degraded mitochondria with obscure cristae in anther cells of M2BS. The results of staining for H2O2 presented a large number of electron dense precipitates (edp) in intercellular space of anther cells of M2BS at anthesis. Moreover, the anther respiration rate and complex I activity of M2BS were significantly lower than those of wild type M2B during pollen development. Furthermore, RNA editing results showed only nad7 presented partially edited at 534th nucleotides. The expression of nad5 and nad7 revealed significant differences between M2B and M2BS.ConclusionsOur data demonstrated that mitochondrial structural degradation and complex I deficiency might be associated with transgenic CMS of rice.
Highlights
Mitochondria play a significant role in plant cytoplasmic male sterility (CMS)
Cytoplasmic male sterility (CMS) was maternally inherited in plants and in most cases originated from mitochondrial DNA rearrangements which resulted in plants inability to produce functional pollen [1,2,3,4,5]
Plant materials M2BS was a transgenic CMS line induced by the partiallength HcPDIL5-2a transformation in rice [16] using M2B as a transgenic receptor material
Summary
Mitochondria play a significant role in plant cytoplasmic male sterility (CMS). Cytoplasmic male sterility (CMS) was maternally inherited in plants and in most cases originated from mitochondrial DNA rearrangements which resulted in plants inability to produce functional pollen [1,2,3,4,5]. CMS produced hybrids using “three-line” system can show heterosis, which exhibits the improved performance of hybrid progeny in comparison with the Generally CMS was associated with mitochondria, which was an essential organelle for cellular energy production [10, 11]. Since pollen development required a large amount of energy supply, disturbances or disorder in mitochondrial functions could have important effects on male fertility [5, 12]. Energy was generated by the production of proton gradient via electron transport, which catalyzed electron transfer from NADH or FADH2 to molecular
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