Abstract
A variety of sugars were found to be subject to degradation during aqueous chromatography at 60°C on lead-form, divinylbenzene—cross—linked sulfonated polystyrene resins. This phenomenon results in only minor interference with the analysis of 2-hexuloses, but presents serious problems in the analysis of 2-pentuloses and prohibitive problems in the analysis of trioses at 60°C. No such problems were encountered during the chromatography of these sugars on the calcium form of the resin under the same conditions. The calcium-form resin is, therefore, the packing material of choice for analysis of 2-pentuloses and trioses, and is to be preferred in the analysis of samples containing 2-hexuloses, unless overriding considerations of selectivity call for use of the lead-form column.
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