Abstract
The fate of isoproturon {N,N-dimethyl-N′-[4-(1-methylethyl)phenyl]urea} in biobeds with and without inoculation with the white rot fungus Phanerochaete chrysosporium was studied. Total extractable isoproturon, its metabolites and formation of non-extractable residues were evaluated. Studies with 14C-isoproturon were also included. A strong decrease in isoproturon was observed in non-inoculated biobeds. Total extractable isoproturon decreased by 76% after 100 days. The decrease was even larger in biobeds inoculated with the white rot fungus P. chrysosporium. After 28 days, total extractable isoproturon decreased by 78%, and after 100 days >99% had disappeared in the inoculated biobeds. However, the studies with 14C-isoproturon showed that 30% of the initially recovered 14C-isoproturon remained in the non-inoculated biobeds as non-extractable residues. As no studies with 14C-isoproturon were performed in inoculated biobeds, it is unclear if the higher rate of disappearance was due to higher biodegradation or higher formation of bound residues.
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