Abstract

Abstract Organisms capable of degrading heterocylic bases have been isolated from soil by enrichment culture techniques and identified as Nocardia species. The mixed biomass of Nocardia species degrades pyridine, picolines, lutidine and quinoline individually as well as in mixture. In a mixture degradation follows a sequential removal of quinoline, alpha‐picoline, 2,6‐lutidine, pyridine, and beta‐picoline. Chemical compositional characteristics of mixed biomass shows chemical formulae of C5H9O2N for a typical microbiological entity. Results on optimisation of environmental conditions for the mixed biomass shows favourable of pH 7.5 and optimum temperature of 30°C for efficent degradation of heterocylic bases. Biokinetic growth constants have been evaluated by shake flask batch experiments for generation of design co‐efficients for laboratory model biological reactor for degradation of these constituents.

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