Degradation of heparan sulfate with heparin lyases.

Abstract

AbstractGlycosaminoglycan (GAG), heparan sulfate (HS), and heparin are a polydisperse mixture of linear polysaccharides composed of glucosamine residues 1→ 4 linked to uronic acid residues. The major repeating unit in heparin is → 4)-α-D-N-sulfoglucosamine-6-sulfate (1? 4)-α-L-iduronic acid-2-sulfate (1?, corresponds to 75-90% of its sequence (1) (see Fig. 1A), whereas heparan sulfate consists of 50-75% ? 4)α-D-N-acetylglucosamine (1? 4)-β-glucuronic acid (1? and smaller amounts of → 4)-α-D-N-acetylglucosamine-6-sulfate (1? 4)-β-D-glucuronic acid (1? and ? 4)α-D-N-sulfoglucosamine (1? 4)-β-D-glucuronic acid (1? (see Fig. 1B). Heparin, which contains approx 2.7 sulfate groups per disaccharide unit, is more highly sulfated than HS, which contains less than one sulfate per disaccharide unit.KeywordsHeparan SulfateDisaccharide UnitHeparan Sulfate ChainDibasic Sodium PhosphateCentrifugal Filter UnitThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.