Abstract

In order to study age related changes in photographic gelatin toe analysed black-and-white roll film before and after accelerated ageing and samples of naturally aged glass plate negatives. Two-dimensional gel electrophoresis (2D-PACE) was used to separate collagen chains In the gelatin by isoelectric point (pi) and molecular weight. The results shoiv that the x-chatns break down into smaller fragments on ageing. Amino acid analysis ivas performed to examine the oxidative deterioration of gelatin. It shows that the amount of lysine decreases with age.

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