Abstract
An endo-polygalacturonase was purified from a commercial preparation of Aspergillus by cation exchange and size exclusion chromatographies. It degraded a polysaccharide extracted from under-retted flax into three general size classes. The first product was a polysaccharide of high molecular weight (close to 100 000), enriched in galactose and characterized by a ratio of galacturonic acid:rhamnose 2:1. The second product was an acidic polysaccharide (the molecular weight of which was close to 10 000) enriched in glucose and rhamnose, which reacted little with the usual colorimetric methods for uronic acids and gave a negative circular dicroism bond. The last and major products were oligomers rich in galacturonic acids.
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