Abstract
The kinetic parameters of isoprene biodegradation were studied in a bioreactor, comprising of bioscrubber and polyurethane foam packed biofilter in series and inoculated with Pseudomonas sp., using a Michaelis–Menten type model. The maximum elimination capacity, ECmax; substrate constant, Ks and ECmax/Ks values for bioscrubber were found to be 666.7gm−3h−1, 9.86gm−3 and 67.56h−1, respectively while those for biofilter were 3333gm−3h−1, 13.96gm−3 and 238.7h−1, respectively. The biofilter section exhibited better degradation efficiency compared to the bioscrubber unit. Around 62–75% of the feed isoprene got converted to carbon dioxide, indicating the efficient capability of bacteria to mineralize isoprene. The FTIR and GC–MS analyses of degradation products indicated oxidative cleavage of unsaturated bond of isoprene. These results were used for proposing a plausible degradation pathway for isoprene.
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