Degradation characteristics of isolated and in situ cell wall lucerne pectic polysaccharides by mixed ruminal microbes

Abstract

AbstractTwo pectic polysaccharide fractions were isolated form lucerne (Medicago sativa L) leaves and used in fermentation experiments with mixed ruminal microbes. Both fractions were similar in chemical composition, containing galacturonic acid (52‐58 mol%) and the neutral sugars arabinose (14‐18 mol%), galactose (6‐8 mol%) and rhamnose (8‐12 mol%). Fermentation of both fractions was rapid and complete with a half‐life of approximately 4 h. Production of total volatile fatty acids matched the degradation profile reaching a maximum level shortly after the rate of degradation began to decrease. The fermentation characteristics of citrus pectin and polygalacturonic acid were similar to those of the lucerne pectic fractions but galacturonic acid was much slower in its rate of degradation while soluble arabinogalactan from larchwood was virtually undegraded. Leaves of early bud stage lucerne and lower nodes and internodes of stems from full bloom lucerne were also fermented by mixed ruminal microbes. Pectic polysaccharides were rapidly and extensively degraded from both tissues. Initial rates were faster for leaves than for stems and the extent of pectic degradation was greater in leaves (8% residual) than in stems (17% residual). Selection of forage lines with increased pectic polysaccharides would provide greater amounts of rapidly available energy that could result in more efficient utilisation of the rapidly degraded protein in lucerne.