Abstract

High-effective decolorization microbial consortium was isolated and selected by enrichment and optimizing combination decolorization experiments. The optimal microbial consortium consisting of a fungus P6 and a bacterium H5 could use Direct Fast Scarlet 4BS as sole source of carbon and energy. The microbial consortium showed a significant improvement on dye decolorization rate under shaking culture that might be attributed to the synergetic reaction of single strains. This study also examined the kinetics of 4BS decolorization by immobilized microbial consortium. The results demonstrated that the activity of microorganisms inside the immobilized beads can be increased remarkably and the dependence of specific decolorization rate on 4BS concentration could be described as a typical first-order reaction kinetic model. The changes of proliferation and distribution of microbial consortium in gel beads were microscopically observed, which could be used for analysis of decolorization mechanism of 4BS.

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