Defining phytochemical phenotypes: size and shape analysis of phenolic compounds in oaks (Fagaceae, Quercus) of the Chihuahuan Desert

Abstract

Interspecific variation in phenolic metabolism across plant species has been correlated to numerous ecological factors, yet generalities concerning the ecological role(s) of phenolics remain elusive. Moreover, studies of allometric variation (absolute and relative concentration) in phenolic metabolism are rare despite the importance of each to ecological interactions. In this study, we characterize individual phenolics in a group of 12 oak species from the Chihuahuan Desert and examine interspecific variation in the absolute and relative concentrations of phenolics using size and shape analysis. Size and shape analysis was able to successfully identify those compounds that contribute most to the interspecific allometric variation in phenolics and classify the oak species on the basis of phenolic metabolism. White versus black oak subgenera were found to be most different in their phenolic composition, where the two black oak species contained fewer and less diverse phenolics. Within the 10 white oak species, a predominantly eastern white oak, Quercus muhlenbergii Engelmann, was found to vary significantly from the more widespread white oaks of the Chihuahuan Desert. We also report the occurrence of complex tannins in oaks of North America for the first time, update proposed pathways of ellagitannin biosynthesis in oaks, and discuss the applicability of size and shape analysis to ecological studies of phenolics.