Abstract

Nepeta viscida and N. nuda subsp. nuda and N. × tmolea were examined in this study. Mainly fresh leaf pieces, dried with silica grains, were used for DNA extraction procedures via DNA isolation kits. Standard PCR techniques were executed using three different primer sets (one nuclear DNA region (nrITS) and two chloroplast DNA regions (rpl32-trnL and trnA(Leu)-trnA(Phe)-trnL-F). DNA sequences were analysed and evaluated using different molecular approaches and software. Consequently, the inconstant molecular structure and hybrid nature of N. × tmolea specimens were shown and interpreted in this study. According to our result, N. × tmolea have some intermediate characters compared to its parents. nrITS data give more information phylogenetically, and also the most polymorphic loci are seen in nrITS data. Morphological and molecular data contribute to define separation of N. × tmolea. Consequently, the inconstant molecular structure and hybrid nature of N. × tmolea specimens were shown and interpreted in this study.

Highlights

  • Lamiaceae family – the mint family – members are well known for their medicinal and aromatic properties in the pharmaceutical industry

  • According to the Flora of Turkey, N. viscida and N. nuda belong to Group A along with N. cataria L., N. isaurica Boiss. & Heldr. ex Benth. and N. caeserea Boiss

  • Stated that N. × tmolea is quite different from its parents but micromorphologically more similar to N. nuda subsp. nuda

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Summary

Introduction

Lamiaceae family – the mint family – members are well known for their medicinal and aromatic properties in the pharmaceutical industry. Recent studies, Turkish Nepeta members have been represented by 44 species. Twentytwo of these species are endemic to Turkey (Aytaç and Yıldız 1996; Güner et al 2000; Dirmenci 2003) with the distribution areas of the species being mainly in east Anatolia and the Taurus Mountains in Turkey (Dirmenci 2005). Nepeta viscida is readily separated from N. nuda subsp. Nepeta viscida is readily separated from N. nuda subsp. nuda by its viscous glandular trichomes and general habit

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