Abstract
Metabolomics uses advanced analytical chemistry methods to analyze metabolites in biological samples. The most intensively studied samples are blood and its liquid components: plasma and serum. Armed with advanced equipment and progressive software solutions, the scientific community has shown that small molecules’ roles in living systems are not limited to traditional “building blocks” or “just fuel” for cellular energy. As a result, the conclusions based on studying the metabolome are finding practical reflection in molecular medicine and a better understanding of fundamental biochemical processes in living systems. This review is not a detailed protocol of metabolomic analysis. However, it should support the reader with information about the achievements in the whole process of metabolic exploration of human plasma and serum using mass spectrometry combined with gas chromatography.
Highlights
Unless the primary plasma preparation looks more attractive: the processing is more reproducible and fast since there is no need to wait for the blood to clot, and clotting time may vary across individuals [37]
This technique becoming the new frontier of gas chromatography-mass spectrometry tandem (GC-MS)-based metabolomics is used to characterize several classes of chemical compounds for panoramic and targeted studies of various biological samples, including blood plasma and serum
We have described each of these stages below (Figure 3)
Summary
Metabolites are substances with low molecular weight (
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