Deficit irrigation interacting with biochar mitigates N2O emissions from farmland in a wheat–maize rotation system

Abstract

Biochar application to agricultural fields is an effective carbon sequestration measure that has the potential to reduce N2O emissions and increase soil water holding capacity. However, the interaction mechanisms of biochar under deficit irrigation on N2O emissions remain unclear. A two-year field experiment is conducted in the Guanzhong Plain, China, in order to quantify the effects of biochar and deficit irrigation on N2O emissions from winter wheat–summer maize crop rotation and to investigate the potential mechanisms of nitrification and denitrification. According to the combination of biochar application and actual evapotranspiration-based irrigation scheduling, four treatments are designed (B1W100: biochar 30 t·ha−1 + ET; B1W80: biochar 30 t·ha−1+ 0.8 ET; B0W100: no biochar + ET; B0W80: no biochar + 0.8ET). The soil N2O flux, soil physical and chemical properties, and key functional gene abundance related to N2O emissions in nitrification and denitrification at different growth stages are investigated and discussed. Results show that the interaction between deficit irrigation and biochar significantly reduces soil N2O emissions. During the wheat and maize season, the application of biochar reduces the N2O emissions by an average of 12.9% and 15.2%, respectively. Deficit irrigation also reduces the N2O emissions by an average of 17.4% and 15.5%, respectively. Pearson correlation analysis shows that soil N2O is significantly correlated with soil water-filled pore space during the phase with intense N2O emissions. Soil functional gene abundance is determined at different growth stages for both wheat and maize. Maximum soil denitrification functional gene abundance is observed at the time when wheat and maize enter the stage of their peak growth at the jointing stage. With biochar addition and deficit irrigation, the abundance of nirK and nosZ genes increases and AOB amoA genes decreases. These results suggest that biochar with deficit irrigation is a better solution to reduce N2O emissions from agricultural soils.