Abstract
BackgroundDengue is a re-emerging public health problem and mosquito-borne infectious disease that is transmitted mainly by Aedes aegypti and Ae. albopictus. Early diagnosis, isolation, and treatment of patients are critical steps for dengue epidemic control, especially to prevent secondary transmission of dengue virus (DENV). However, little is known about defervescent dengue patients as a source of infection.MethodsThis case study describes 1268 dengue patients hospitalized at Guangzhou Eighth People’s Hospital from June 2013 to December 2014. The viral loads of each individual were measured using real-time reverse transcription-polymerase chain reaction. Ae. aegypti and Ae. albopictus were exposed to blood meal with gradated dengue viral loads to characterize the relationship between viremia in dengue patients and the vector competence of vector mosquitoes.ResultsThe viral numbers in the blood were measured, ranging from 108 to 103 copies/ml from day 1 to day 12 after fever onset. Vector competence analysis of Ae. aegypti and Ae. albopictus indicated that viremia > 104 copies/ml can still infect vector mosquitoes, which implied that the defervescent dengue patients might be a source of infection.ConclusionsThe results of this study indicate that some defervescent dengue patients still have sufficient viral load to infect vector mosquitoes. Therefore, the protection against mosquito biting for these people should be extended to prevent secondary transmission events.
Highlights
Dengue is a re-emerging public health problem and mosquito-borne infectious disease that is transmitted mainly by Aedes aegypti and Ae. albopictus
Is it possible for defervescent dengue patients to be a source of infection? What is the changing role of viremias in dengue patients after fever onset? Is there any minimum dengue virus (DENV) copy number required to infect vector mosquitoes? To answer these questions, we analyzed the viremias in 1268 dengue patients ranging from
The percentage of patients who were positive for DENV RNA declined from 100% on day 1 to 23.53% on day 12 after disease onset
Summary
Dengue is a re-emerging public health problem and mosquito-borne infectious disease that is transmitted mainly by Aedes aegypti and Ae. albopictus. Little is known about defervescent dengue patients as a source of infection. Dengue is a mosquito-borne infectious disease and is transmitted mainly by Aedes aegypti and Ae. albopictus. The host and virus variables are associated with DENV transmission from symptomatic dengue cases to vector mosquitoes [3]. Is it possible for defervescent dengue patients to be a source of infection? What is the changing role of viremias in dengue patients after fever onset? We analyzed the viremias in 1268 dengue patients ranging from (2020) 9:17 day 1 to day 12 after fever onset and tested the infection rates of various viral loads of DENV in blood meals on vector mosquitoes Is it possible for defervescent dengue patients to be a source of infection? What is the changing role of viremias in dengue patients after fever onset? Is there any minimum DENV copy number required to infect vector mosquitoes? To answer these questions, we analyzed the viremias in 1268 dengue patients ranging from (2020) 9:17 day 1 to day 12 after fever onset and tested the infection rates of various viral loads of DENV in blood meals on vector mosquitoes
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