Defense strategies in Cenchrus biflorus, C. ciliaris, and C. orientalis to cope with aridity, salinity, and cold stress

Abstract

Three Cenchrus species (C. biflorus, C. ciliaris, and C. orientalis) were collected from different ecological regions in the Punjab and Khyber Pakhtoonkhwa during July to September 2020 to evaluate tolerance to multiple abiotic stresses (aridity, salinity, and cold). Growth and biomass production was the maximum in C. biflorus, C. ciliaris, and C. orientalis under aridity stress. Aerenchyma formation and vascular tissue increased significantly in roots under aridity. In C. ciliaris, aerenchyma formation was recorded under cold stress. Root vascular tissue was higher under normal conditions (26.7%) and aridity (30.4%) stress in C. ciliaris. Stem parenchyma (29.1%) and vascular tissues (35.5%) showed an increase in C. biflorus under aridity, while mechanical tissue (33.6%) increased significantly under salinity stress. In C. biflorus and C. ciliaris, extensive sclerification was recorded in the stelar region under aridity stress. The stem radius of C. orientalis was greatest under salinity (27.0%) and cold (29.1%) stress, while parenchyma tissue was smaller (12.1%) under cold stress. Leaf thickness generally increased under abiotic stresses, and the maximum (43.0%) thickness was recorded in C. biflorus under aridity stress. Cenchrus biflorus, C. ciliaris, and C. orientalis showed strong association among morphological and leaf anatomical traits under aridity stress. Cenchrus orientalis showed high sclerification outside the vascular bundles of the leaf sheath. In saline conditions, vascular bundles were sclerified and enlarged. In and outside the vascular bundles of leaf blades, extensive sclerification was observed under cold stress conditions. Modifications in structural and functional traits were critical for the ecological success of Cenchrus species for survival in multiple environmental stresses.