Defense responses in tomato fruit induced by oligandrin against Botrytis cinerea

Abstract

Oligandrin is known to induce resistance against a number of plant diseases. However, its effects on postharvest diseases are still unclear. The effects of oligandrin on the control of postharvest diseases in tomato fruit and its underlying mechanisms were investigated in this study. The treatment of tomato fruit with oligandrin (10 μg/ml) significantly reduced the incidence and severity of gray mould (caused by Botrytis cinerea). After 5, 7 and 9 days of artificial inoculation, the relative cure effect was 60.5, 52.1 and 48.5%, respectively. The results from bio-assay indicated that the treatment stimulated the activity of the defense related enzymes. Phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) activity in the oligandrin-treated fruit was about 39.2, 69.6 and 52.6% higher than that in control on the 3rd day, respectively. Furthermore, mRNA level of the genes encoding pathogenesis-related proteins (PRs), such as PR-2a(extracellular β-1, 3 -glucanase) and PR-3a (extracellular chitinase) in tomato fruit was about 2.7-fold and 4.6-fold above that of the control at the peak stage. The expression of LeERF2 and PR6, which confer an ethylene-dependent signaling pathway, were also significantly increased 6.6-fold and 3.6-fold by such treatment. These results indicate that oligandrin has the potential to control gray mould and it may play an important role in the induction of resistance to B. cinerea and the activation of the ethylene-dependent signaling pathway. Key words: Tomato, disease resistance, oligandrin, Botrytis cinerea.