Abstract
Planting resistant cultivars is the most effective tactic to manage the thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) in peanut plants. However, molecular mechanisms conferring resistance to TSWV in resistant cultivars are unknown. In this study, transcriptomes of TSWV-susceptible (SunOleic 97R) and field-resistant (Tifguard) peanut cultivars with and without TSWV infection were assembled and differentially expressed genes (DEGs) were compared. There were 4605 and 2579 significant DEGs in SunOleic 97R and Tifguard, respectively. Despite the lower number of DEGs in Tifguard, an increased proportion of defense-related genes were upregulated in Tifguard than in the susceptible cultivar. Examples included disease resistance (R) proteins, leucine-rich repeats, stilbene synthase, dicer, and calmodulin. Pathway analysis revealed the increased downregulation of genes associated with defense and photosynthesis in the susceptible cultivar rather than in the resistant cultivar. These results suggest that essential physiological functions were less perturbed in the resistant cultivar than in the susceptible cultivar and that the defense response following TSWV infection was more robust in the resistant cultivar than in the susceptible cultivar.
Highlights
Peanut (Arachis hypogaea L.) is an important crop for oil and protein production [1].Spotted wilt disease caused by thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) severely affects peanut production in southeastern United States [2,3]
97R as opposed to the resistant cultivar Tifguard. These results suggest that, in addition to having a robust defense system, the essential functions associated with plant growth and photosynthesis were less impacted due to TSWV infection in the resistant cultivar rather than in the susceptible cultivar
TSWV-resistant cultivars have been widely used across southeastern United States and are pivotal for peanut production
Summary
Spotted wilt disease caused by thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) severely affects peanut production in southeastern United States [2,3]. In Georgia alone, spotted wilt disease causes an annual loss of over USD 10 million [4,5,6]. Tomato spotted wilt orthotospovirus is the species type of the genus Orthotospovirus in the family Tospoviridae. TSWV is an ambisense RNA virus that is exclusively transmitted in a persistent and propagative fashion by the peanut-colonizing thrips species in the family Thripidae [7,8,9]. Frankliniella fusca [Hinds], and western flower thrips, Frankliniella occidentalis [Pergande], are the predominant vectors of TSWV in southeastern United States [5,10]
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