Abstract
ABSTRACT. Replication-defective avian sarcoma viruses can only propagate in tissue culture when the defective function is complemented by a helper virus or helper cell thus complicating genetic and biochemical analysis of the defective particle. This complication can be overcome by developing transformed avian cell strains which produce large quantities of cloned, defective virus particles during many cell generations. This study describes the establishment of transformed quail and turkey cell strains with the Bryan high titer strain of Rous sarcoma virus. The biological and biochemical properties of the defective particles elaborated by these cells are studied. Two of the transformed cell strains produce particles similar to variants of the Bryan virus previously described, namely BH-RSV(-) and BH-RSVα. A new class of Bryan replication defective virus is produced by a transformed turkey cell strain. This virus carries genetic information for the subgroup specificity of the major envelope glycoprotein but lacks a functional reverse transcriptase. Complementation or recombination readily occurs between this virus and clones that are defective for envelope glycoprotein but code for reverse transcriptase.
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