Abstract

Functional complementation among “middle,” “bottom,” and unstable variant components of strains of tobacco rattle virus was followed using genetic markers, in terms of differences in symptomatology and particle lengths, that occur in three strains derived from a single source. Complementation was not rigorously strain specific, and occurred between appropriate components of the same and different strains. Artificial “hybrid” strains thus made had the symptomatology and particle-length heterogeneity characteristic of the strain from which the middle component used originated. The results confirm that the components of tobacco rattle viruses originate separately, are functionally specialized, and are interdependent for functions which they lack.

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