Abstract
Background/Aims: Platelet function abnormalities contribute to the hemostatic defect in patients with cirrhosis. In this study we evaluated the occurrence of in vivo platelet activation as a possible mechanism of defective platelet aggregation in patients with cirrhosis. Methods: Nine patients with severe (Child B-C) cirrhosis and defective platelet aggregation were studied in comparison with age- and sex-matched healthy controls. The presence of activated platelets in the bloodstream was evaluated by fluorescence-activated flow cytometry using antibodies directed against activation-dependent platelet proteins and by measuring plasma levels of β-thromboglobulin and platelet factor 4. Urinary levels of 11-dehydro-TXB 2 and of 2,3-dinor-TXB 2 were assayed by radioimmunoassay following chromatographic separation. Results: In unstimulated platelets, the expression of both GMP 140 and GP 53 was not significantly different in patients with cirrhosis and in controls. After stimulation with ADP and epinephrine, expression of activation-dependent antigens was lower in platelets from patients (GMP 140: 0.64±0.09 vs 0.73±0.04, p=0.02; GP 53: 0.41±0.13 vs 0.54±0.14). Plasma levels of β-thromboglobulin and platelet factor 4, as indexes of in vivo platelet activation, were also comparable in the two groups of subjects. Urinary levels of 11-dehydro-TXB 2 and of 2,3-dinor-TXB 2, the major systemic metabolites of TXA 2, were significantly higher in patients with cirrhosis (1807±518 vs 341±121 ng/pg creatinine and 693±512 vs 205 (93 ng/pg creatinine, respectively, p<0.001). However, increased excretion of TXB 2 metabolites was also observed in three patients with chronic autoimmune thrombocytopenia. Conclusions: These data indicate that circulating platelets are not activated in cirrhosis, and that defective aggregation is most likely dependent on the alteration of the transmembrane signaling pathways. The increased urinary excretion of systemic TXA 2 metabolites may be related to increased intrasplenic platelet destruction.
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