Abstract
The differences in the intracellular Ca<sup>2+</sup> responses to hormones in platelets from systemic lupus erythematosus (SLE) patients compared to normal humans have not been explored. This study examined the Ca<sup>2+</sup> signaling and density of platelets in normal, inactive and active SLE patients. The platelet number per µl in inactive and normal groups did not differ, whereas the number in active SLE patients was smaller than the other two groups by 60%. The intracellular free Ca<sup>2+</sup> levels ([Ca<sup>2+</sup>]<sub>i</sub>) in response to stimulation of four endogenous Ca<sup>2+</sup> mobilizing hormones, 100 µM arachidonic acid (AA), 10 µM ADP, 10 nM platelet activation factor (PAF) and 1 µM thrombin, were investigated using the Ca<sup>2+</sup>-sensitive fluorescent dye, fura-2. The AA-induced [Ca<sup>2+</sup>]<sub>i</sub> rises in normal and inactive groups were similar. In contrast, the AA-induced [Ca<sup>2+</sup>]<sub>i</sub> rises in the active SLE group were significantly smaller than in the normal and inactive groups. The defect in the AA-induced [Ca<sup>2+</sup>]<sub>i</sub> rises in active SLE groups appears to be caused by defective Ca<sup>2+</sup> influx and Ca<sup>2+</sup> releasing pathways because the AA-induced responses were not altered by removal of extracellular Ca<sup>2+</sup>, whereas the AA-induced responses in normal and inactive SLE groups were reduced by removal of extracellular Ca<sup>2+</sup>, and the AA-induced Ca<sup>2+</sup> release was smaller in the active SLE group. PAF, ADP and thrombin all induced [Ca<sup>2+</sup>]<sub>i</sub> rises in the three groups, but no significant differences were found among the three groups. Together, the results indicate that cell density and Ca<sup>2+</sup> signaling in platelets from active SLE patients are altered in response to particular stimulators. In these regards, platelets from inactive SLE patients appear to be similar to those from normal humans.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.