DeePromClass: Delineator for Eukaryotic Core Promoters Employing Deep Neural Networks.

Abstract

Computational promoter identification in eukaryotes is a classical biological problem that should be refurbished with the availability of an avalanche of experimental data and emerging deep learning technologies. The current knowledge indicates that eukaryotic core promoters display multifarious signals such as TATA-Box, Inr element, TCT, and Pause-button, etc., and structural motifs such as G-quadruplexes. In the present study, we combined the power of deep learning with a plethora of promoter motifs to delineate promoter and non-promoters gleaned from the statistical properties of DNA sequence arrangement. To this end, we implemented convolutional neural network (CNN) and long short-term memory (LSTM) recurrent neural network architecture for five model systems with [-100 to +50] segments relative to the transcription start site being the core promoter. Unlike previous state-of-the-art tools, which furnish a binary decision of promoter or non-promoter, we classify a chunk of 151mer sequence into a promoter along with the consensus signal type or a non-promoter. The combined CNN-LSTM model; we call "DeePromClass", achieved testing accuracy of 90.6%, 93.6%, 91.8%, 86.5%, and 84.0% for S. cerevisiae, C. elegans, D. melanogaster, Mus musculus, and Homo sapiens respectively. In total, our tool provides an insightful update on next-generation promoter prediction tools for promoter biologists.