Deeper investigation of oxygen-containing compounds in oleaginous feedstock (animal fat) by preparative column chromatography and comprehensive two-dimensional gas chromatography coupled with high-resolution time-of-flight mass spectrometry

Abstract

The production of renewable fuels as biodiesel and bio-jet fuel is usually originated by the transformation and processing of oleaginous feedstocks, mainly composed of triacylglycerols. Currently, a significant part of the triacylglycerol production relies on grassy oil crops or other woody oil plants, representing more than 120 million metric tons every year. Considering that the worldwide triacylglycerol demand is expected to rise in the future, alternative routes are necessary to ensure a sustainable biodiesel industry and limit diesel price volatility. In this context, the use of animal fats could be an interesting alternative for biodiesel production as the production of animal byproducts represents nearly 17 million tons per year in the European Union only (2020). Animal fats, however, contain large amounts of no-esterified fatty acids and other oxygen compounds, reducing the yield of biodiesel. Therefore, a specific pretreatment is needed before the trans-esterification process. The setup of such appropriate pretreatments requires detailed upstream characterization of the minor components present in the feedstock. For this purpose, the minor component profile of animal fat was investigated by comprehensive two-dimensional gas chromatography coupled with high-resolution time-of-flight mass spectrometry. This was preceded by an innovative sample fractionation and focalization of these minor components by a preparative liquid chromatographic column method. The overall method permitted to extract different levels of information from the two-dimensional chromatograms, leading to a tentative identification of more than 150 compounds, mainly oxygenated, belonging to different chemical classes.