Deep soil water extraction by apple sequesters organic carbon via root biomass rather than altering soil organic carbon content

Abstract

Soil water and carbon stocks have always been research hotspots. However, the interaction between soil water and carbon in deep soil (>1 m below surface) remains poorly understood. The present study used the chronosequence approach to investigate water extraction and carbon input by roots to a depth of 25.2 m in 8-, 11-, 15-, 18-, and 22-year-old afforested apple (Malus pumila Mill.) orchard stands in a sub-humid region of the Chinese Loess Plateau. Three long-term cultivated farmlands were used as a benchmark of soil water and carbon status before land use change. Measurements showed that the apple trees accessed deep soil water reserves by growing deep roots, with the resulting desiccated soil possibly stimulating apple trees to extend their roots into deeper, moister soil. Accordingly, soil water content in the root zone decreased progressively with increasing stand age. For example, the roots of apple trees in the 22-year-old stand extended to 23.2 m below the soil surface and extracted 1530 ± 43 mm deep soil water. Consequently, carbon input from root biomass correlated well with the water storage loss in deep soil (R2 = 0.88). Deep roots accounted for 49 ± 22% of the total root biomass and contributed 0.44 ± 0.15 Mg C ha−1 yr−1 to the deep soil. However, the roots of apple trees did not significantly change the soil organic carbon content in the root zone possibly because there was limited root biomass per unit soil depth and because soil water content in the root zone gradually decreased. These findings demonstrate the importance of deep soil in regulating water and carbon cycles, advancing our understanding of interactions among water, roots, and carbon in this zone.