Abstract
Abstract Maintenance of peripheral tolerance requires a balance between auto-reactive conventional T cells (Tconv) and thymically-derived Foxp3+ regulatory T cells (tTregs). Considerable controversy exists regarding the similarities/differences in TCR repertoires expressed by Tconv and tTregs. We generated highly purified populations of human Tconv and tTregs based on the differential expression of CD25 and CD127. The purity of the sorted populations was validated by intracellular staining for Foxp3 and Helios. We used deep sequencing of entire TCRB sequences to analyze the TCR repertoire of Tconv and tTregs. Our studies demonstrate that Tconv and tTreg cells are, in fact, entirely distinct CD4+ T cell lineages.
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