Deep sequencing of the microRNA expression in fall dormant and non-dormant alfalfa.

Wenna Fan,Pengfei Shi,Chengzhang Wang

doi:10.1016/j.gdata.2014.09.007

Abstract

MicroRNAs (miRNAs) play a critical role in post-transcriptional gene regulation that down-regulates target genes by mRNA degradation or translational repression. Evidence is increasing for their crucial roles during plant development. Identification of miRNAs at the global genome-level by high-throughput sequencing is essential to functionally characterize miRNAs in plants. Alfalfa (Medicago sativa L.) is one of the most widely cultivated perennial forage legumes worldwide. Fall dormancy is an adaptive character related to the biomass production and winter survival in alfalfa. However, little is known about miRNA-mediated developmental regulation of fall dormancy in alfalfa. Here, we provide detailed experimental methods and analysis pipeline in our study to identify miRNAs that were responsive to fall dormancy (Fan W et al., Genome-wide identification of different dormant Medicago sativa L. microRNAs in response to fall dormancy, submitted for publication) for reproducible research. The data generated in our work provide meaningful information for understanding the roles of miRNAs in response to seasonal change and growth regulation in alfalfa.

Highlights

Data in BriefDeep sequencing of the microRNA expression in fall dormant and non-dormant alfalfa
MicroRNAs play a critical role in post-transcriptional gene regulation that down-regulates target genes by mRNA degradation or translational repression
The data generated in our work provide meaningful information for understanding the roles of miRNAs in response to seasonal change and growth regulation in alfalfa

Summary

Data in Brief

Deep sequencing of the microRNA expression in fall dormant and non-dormant alfalfa. Little is known about miRNA-mediated developmental regulation of fall dormancy in alfalfa. We provide detailed experimental methods and analysis pipeline in our study to identify miRNAs that were responsive to fall dormancy The data generated in our work provide meaningful information for understanding the roles of miRNAs in response to seasonal change and growth regulation in alfalfa. Medicago sativa L. standard varieties Maverick and CUF101 N/A Illumina Hiseq 2000 Raw data: FASTQ files Fall dormant vs nondormant Small RNA sequencing for miRNA expression analysis in fall dormant and fall non-dormant alfalfa leaves N/A The Experimental Station of Henan Agricultural University, Zhengzhou, China (34°19 × N, 113°35 × E)

Direct link to deposited data

Experimental design

Plant materials and total RNA isolation

Generation of sequencing data

MiRNAs expression analyses

Discussion