Deep phylogeographic break among Octopus variabilis populations in China: Evidence from mitochondrial and nuclear DNA analyses

Abstract

The genetic structure of marine populations is greatly influenced by the dispersal ability of the organisms, and it is intuitive that a limited dispersal capability would result in greater genetic differentiation. Octopus variabilis is a typical cephalopod species that shows limited dispersal potential, and previous genetic surveys by a mitochondrial DNA analyses revealed high levels of genetic differentiation among its populations, suggesting the existence of cryptic or subspecies. To test this hypothesis, amplified fragment length polymorphisms (AFLPs) and the cytochrome oxidase II (COII) gene sequence were used to re-evaluate the spatial genetic structure of five populations of O. variabilis along the coast of China. Four distinct groups were identified within these five populations: the Dalian + Qingdao, Zhoushan, Wenzhou, and Dongshan groups. A deep genetic break among the Dongshan, Wenzhou, and other two groups were specifically recognized by both genetic markers. A considerable number of distinct alleles for AFLP and a deep divergence of 13.9–19.4% in the COII gene sequences were found among them, suggesting the occurrence of potential cryptic or subspecies of O. variabilis. A genetic structure of isolation by distance was identified in O. variabilis by a Mantel test, with the geographic distance explaining 62% of the variation in genetic differentiation. This result suggests that gene flow is geographically restricted for this species and that limited dispersal may be the main reason for the genetic differentiation among O. variabilis populations. The present results indicate that special care should be taken in future fishery exploitation and conservation efforts for this species and that conservation management should include populations representing all lineages.