Abstract
Intracellular processes such as cytoskeletal organization and organelle dynamics exhibit massive subcellular heterogeneity. Although recent advances in fluorescence microscopy allow researchers to acquire an unprecedented amount of live cell image data at high spatiotemporal resolutions, the traditional ensemble-averaging of uncharacterized subcellular heterogeneity could mask important activities. Moreover, the curse of dimensionality of these complex dynamic datasets prevents access to critical mechanistic details of subcellular processes.
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