Abstract

In this paper we focus on the role of deep instance segmentation of laboratory rodents in thermal images. Thermal imaging is very suitable to observe the behaviour of laboratory animals, especially in low light conditions. It is an non-intrusive method allowing to monitor the activity of animals and potentially observe some physiological changes expressed in dynamic thermal patterns. The analysis of the recorded sequence of thermal images requires smart algorithms for automatic processing of millions of thermal frames. Instance image segmentation allows to extract each animal from a frame and track its activity and thermal patterns. In this work, we adopted two instance segmentation algorithms, i.e., Mask R-CNN and TensorMask. Both methods in different configurations were applied to a set of thermal sequences, and both achieved high results. The best results were obtained for the TensorMask model, initially pre-trained on visible light images and finally trained on thermal images of rodents. The achieved mean average precision was above 90 percent, which proves that model pre-training on visible images can improve results of thermal image segmentation.

Highlights

  • Laboratory animals’ behavior analysis is often used in studies of stress, anxiety, depression or neurodegenerative diseases [1]

  • We describe the methods for instance segmentation adopted by us for thermal image of laboratory animals

  • In order to compare the effectiveness of both architectures, we performed additional Mask R-CNN training with optimal parameters for TensorMask (2 batches, 100,000 epochs)

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Summary

Introduction

Laboratory animals’ behavior analysis is often used in studies of stress, anxiety, depression or neurodegenerative diseases [1]. The automation of this analysis has undoubtedly many advantages, among which objectivity and standardization are one of the most desirable. The spectrum of behavior analysis performed by the available systems is usually limited to exploration, rest or grooming. All these behaviors are detected based on simple object parameters representing the position, speed, direction of the movement or the shape parameters of the observed rodent [3]. Gentle grooming can be a form of defense, it can be confused with other behavior—climbing

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