Deep Genomic Sequencing of Bladder Urothelial Carcinoma in Southern Chinese Patients: A Single-Center Study.

Dong-Yang Li,Hai-Lun Zhan,Jia-Rong Cai,Wei-Qiang Liao,Bo-Long Liu,Xiang-Fu Zhou,Wen-Biao Li,Yun Luo,Fei Yang

doi:10.3389/fonc.2021.538927

Dong-Yang Li, Hai-Lun Zhan + Show 7 more

Open Access

https://doi.org/10.3389/fonc.2021.538927

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Abstract

ObjectiveBladder urothelial carcinoma (BUC) is a common urological malignancy with molecular heterogeneity. However, the genetic feature of Chinese BUC patients is still not well-identified.MethodsWe performed deep sequencing by a large panel (450 genes) on 22 BUC samples and using matched normal bladder tissue as control. Genomic alterations (GAs), pathways and Tumor Mutation Burden (TMB) were investigated.ResultsThe frequencies of GAs (TERT, 54.5%; CREBBP, 27.3%; GATA3, 22.7%; BRAF, 18.2%; TEK, 18.2% and GLI1, 18.2%) were significantly higher in Chinese than Western BUC patients. Other GAs’ frequencies were in accordance with previous study (TP53, 50.0%; KDM6A, 31.8%; KMT2D, 22.7%; etc.). Besides, we detected gene amplification in ERBB2, FRS2, FAS, etc. The gene fusion/rearrangement took place in the chromosome 11, 12, 14, 17, 19, 22, and Y. Other than cell cycle and PI3K-AKT-mTOR, mutated genes were more associated with the transcription factor, chromatin modification signaling pathways. Interestingly, the TMB value was significantly higher in the BUC patients at stages T1–T2 than T3–T4 (P = 0.025).ConclusionDeep genomic sequencing of BUC can provide new clues on the unique GAs of Chinese patients and assist in therapeutic decision.

Highlights

Bladder cancer (BCa) is a common malignancy among urological cancers, ranking only second to prostate cancer (1)
The tumor mutation burden (TMB) value was significantly higher in the bladder urothelial carcinoma (BUC) patients at stages T1–T2 than T3–T4 (P = 0.025)
Even though traditional treatment strategies such as surgery, Genomic Alterations in Bladder Cancer instillation chemotherapy or vaccine therapy can be applied, up to 70% Non-muscle invasive bladder cancer (NMIBC) will recur within 2 years (5)

Summary

Results

The frequencies of GAs (TERT, 54.5%; CREBBP, 27.3%; GATA3, 22.7%; BRAF, 18.2%; TEK, 18.2% and GLI1, 18.2%) were significantly higher in Chinese than Western BUC patients. Other GAs’ frequencies were in accordance with previous study (TP53, 50.0%; KDM6A, 31.8%; KMT2D, 22.7%; etc.). We detected gene amplification in ERBB2, FRS2, FAS, etc. The gene fusion/rearrangement took place in the chromosome 11, 12, 14, 17, 19, 22, and Y. Other than cell cycle and PI3K-AKTmTOR, mutated genes were more associated with the transcription factor, chromatin modification signaling pathways. The TMB value was significantly higher in the BUC patients at stages T1–T2 than T3–T4 (P = 0.025)

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