Abstract
DE-DIFFERENTIATION OF PAROTID ACINAR CELLS IN VITRO Andrew S. Currie July 16, 2010 Background: When rat salivary gland cells are cultured in vitro, they dedifferentiate within 24 h. Hypothesis: Growth factors will prevent de-differentiation of primary cultures of parotid gland cells, and they will induce differentiation of ParC5 cells. DNA methylation drives de-differentiation and the loss of expression in cultured parotid cells. Methods: Rat parotid glands were collected and cultured in media using different combinations of growth factors. DNA was evaluated for methylation at various time points. Results: No media tested prevented the de-differentiation of parotid cells, and none induced differentiation of ParC5 cells. Limited variation in methylation of CpG sites was seen. Conclusion: Growth factors do not prevent de-differentiation in primary cell culture, nor do they activate differentiation in ParC5 cells. Global methylation of Mist1 does not cause de-differentiation; evidence supports that methylation of PSP increases over time.
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