Decreasing of Crude Fibre in Indigofera Leaves Flour Hydrolysed with Cellulase Enzyme as a Source of Feed Protein

Abstract

The problem of fish feed in Indonesia is currently dominated by import dependence on feed raw materials, especially fish meal and soybean meal. One alternative that currently can be chosen is indigofera leaves (Indigofera zollingeriana). The indigofera leaves used are indigofera leaves that have been cultivated by farmers in Kemiling District, Bandar Lampung City. Harvest is done at the age of 12 months. After harvesting, indigofera leaves are dried in the sun and the flouring process is carried out. Furthermore, indigofera and indigofera leaves were tested proximately and compared with commercial indigofera flour. In addition, the resulting indigofera leaf flour was hydrolyzed with cellulose enzyme at a dose of 10 g/kg for 5 days and a proximate test was carried out afterwards. The results showed leaf protein content of 19.95%, protein content of treated indigofera leaves flour 29.74% while protein content of commercial indigofera flour was 21.95% (dry weight). Meanwhile, the protein content of indigofera leaves flour which had been hydrolyzed with cellulose enzyme at a dose of 10 g/kg for 5 days increased to 31.30% and crude fiber decreased from 28.75% to 1.98%. The conclusion of this study is that the resulting indigofera leaves flour has the lowest crude fiber content and higher protein than indigofera leaves flour without hydrolysis of cellulose enzymes and commercial indigofera flour. Indigofera leaves flour hydrolyzed with cellulose enzymes resulted in a decrease in crude fiber and an increase in protein.