Decreasing mRNA HMGB1 expression in Klebsiella pneumoniae infection treated by Miana (Coleus scutellarioides (L.) Benth): a cohort experimental study.

Abstract

This study comprised a cohort experiment using 20 Balb/c mice divided into four groups. Balb/c mice in each group were intraperitoneally injected with K. pneumoniae. Group 1 was given a placebo; Group 2 was given Miana; Group 3 was given levofloxacin; and Group 4 was given both levofloxacin and Miana. The levels of mRNA HMGB1 expression were measured using real-time PCR before, during, and after the infection as well as after the treatments. The initial examination results showed that the average level of mRNA HMGB1 expression was 5.51fc. The mRNA HMGB1 expression in mice after being challenged with K. pneumoniae was 9.64fc. Group 1 that was given a placebo had a mean mRNA HMGB1 expression level of 14.99fc. Group 2 that was given Miana had a mean mRNA HMGB1 expression level of 13.95fc. Group 3 that was given levofloxacin had an average mRNA HMGB1 expression level of 6.45fc, and Group 4 that was given levofloxacin and Miana together had an average mRNA HMGB1 expression level of 5.59fc. Miana (Coleus scutellarioides (L.) Benth) increased mRNA HMGB1 expression at the initial administration via regulation of the immune system. Administration of Miana following K. pneumoniae infection inhibited the increase in mRNA HMGB1 expression. Treatment with levofloxacin reduced the level of mRNA HMGB1 expression, and the effect was optimized by the administration of Miana leaf extract as a supplement.