Abstract
The surprising resistance of the bacteriophage to action of disinfectants has been commented upon by many observers. Thus d'Herelle1 states that bacteriophage corpuscles survive 3 days'exposure to 0.5% mercuric chloride in physiological saline. Prausnitz and Firle2 claim that resistance is above that of anthrax spores. Watanabe3 noted well defined racial variations in the resistance of'phages to phenol, 1% for 24 hours being sufficient to destroy some races, while 4% was necessary to inactivate others.In order to determine whether the organic matter in the lysates exerts any protective action, 3 races of bacteriophage were purified according to the technique of Arnold and Weiss.4 These purified'phages gave negative Biuret, Xanthoproteic acid, Millon, Hopkins, Cole and Molisch tests. Non-purified'phages were adjusted to the same lytic titer and the lysol resistance of both the purified and non-purified suspensions was compared. Tests of each'phage were made in triplicate at various lysol concentrations. Th...
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